Gene Manipulation and Genetic Engineering (V-U)

• Introduction (V-U)
• What is genetic engineering? (V-U)
• Genes and Genome (V-U)
• The flow of genetic information (V-U)
• Molecular cloning (V-U)
• Basic techniques (V-U)
• Basic Techniques of Gene Manipulation-an overview (V-U)
• Isolation of genomic DNA (V-U)
• Agarose gel electrophoresis (V-U)
• Nucleic acid blotting (V-U)
• Autoradiography (V-U)
• Southern blotting (V-U)
• Northern blotting (V-U)
• Western blotting (V-U)
• Transformation of E. coli , Electroporation (V-U)
• Transformation with other organisms (V-U)
• Polymerase Chain Reaction (PCR) (V-U)
• Host-controlled restriction modification (V-U)
• Types of restriction and modification (R-M) system (V-U)
• Nomenclature of restriction endonucleases (V-U)
• Target sites of restriction endonucleases (V-U)
• Number and size of restriction fragments (V-U)
• Summary of restriction endonucleases (V-U)
• DNA modifying enzymes (V-U)
• Methods of joining DNA fragments (V-U)